Mercoledì 23 Febbraio 2022, ore 13
Edificio Asclepio U8-Aula 8
Seminario in presenza
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Invited Research Meeting – Host: Dott.ssa Maria Grazia Cerrito
Ana Maria de Paula
Physics Department, Federal University of Minas Gerais, Brazil
Multiphoton imaging on cancer biopsies
We present microscopy images of biological tissue by second harmonic generation (SHG) and multiphoton excited fluorescence microscopy as a potential tool in helping with cancer diagnosis. The nonlinear imaging allows us to evaluate the tissue collagen and the cellular regions in the biopsies (example below). We demonstrate a methodology to evaluate the changes caused by cancer in collagen and cellular parameters of histological biopsies using automated image analysis and machine learning techniques.
The procedure allowed to distinguish between the healthy and cancerous tissue with an accuracy of around 90% for canine mammary cancer and for human prostate cancer. In addition the results for canine mammary gland carcinomas show that the measured tissue collagen parameters correlate with the clinical and pathological data, and the dogs survival times.
Ana Maria de Paula obtained her undergraduate in Physics from the Federal University of Minas Gerais (UFMG) Brazil, a Master's degree in Physics (optics) from UFMG and a PhD in Ultrafast spectroscopy in semiconductors from the University of Oxford-UK. She is now an associate professor at the Federal University of Minas Gerais - Brazil, where she leads the multidisciplinary laboratory in ultrafast spectroscopy and nonlinear optical microscopy imaging by second harmonic generation and fluorescence by absorption of two photons applied to nanomaterials and biological materials.